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※納米金棒的合成、性能
※生物醫學領域的應用：醫學成像
※生物醫學領域的應用：光熱反應
※生物醫學領域的應用：靶向治療
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納米金棒的特性: X Huang, S Neretina, M A. El-Sayed. Gold Nanorods: From Synthesis and Properties to Biological and Biomedical Applications. Adv. Mater. 2009, 21, 1–31.
shape. Reproduced with permission from [155]. C) Mechanism tor gold nanorod growth in the presence of silver ions. Ag+ is reduced to Ag0 at a metal substrate with a surface potential less than the standard reduction potential of Ag+, called silver underpotential deposition (UPD). The deposition of silver on the side {110} facet is faster than on the end {100} facets due to lower reduction potential on the {110} facet. This inhibits the nanorod growth on the side face leading to preferential growth of gold at the ends. Increasing the silver concentration results in more silver deposition on the side facet and the nanorod growth of higher aspect ratio. Further increase of the silver concentration results in the silver deposition of whole rods and thus stopping nanorod growth. Reproduced with permission from [163]. Copyright 2006 ACS. |
| 納米金棒最佳尺寸: Mechanism for the Cellular Uptake of Targeted Gold Nanorods of De?ned Aspect Ratios (H Yang, Z Chen, L Zhang el at. Small, 2016, 12, No. 37, 5178–5189)
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※生物醫學領域的應用：醫學成像
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Huang et al. [280] demonstrated that gold nanorods can be used for cancer diagnosis by using anti-EGFR antibodies that bind homogenously and predominately to cancer cells due to EGFR overexpression on such cells (Fig. 16A).
Ding et al.[369] used dark ?eld light scattering to image and monitor the receptor-mediated uptake of nanorods into HeLa cells. Nucleus targeting and imaging have also been achieved by conjugating the nanorods to the NLS (Fig. 16B). [326] |
| gold anorods were conjugated to anti-EGFR anti-bodies enabling selective photothermal therapy due to their preferential binding nto
human oral cancer cells. A CW Ti/Sappire NIR laser with a wavelength at 800 nm, overlapping with the SPR absorption maximum of the gold nanorods (aspect ratio of 3.9), was used for the photoirradiation of the cells immunolabeled with the nanorods.
Using the trypan blue cell death staining and variable laser energies, they found that the cancer cells required half the laser energy (10 W cm-2, Fig. 18A) to be photothermally damaged as compared to the normal cells (20 W cm-2,) (Fig. 18B). In their recent studies using a mouse model, [270] the nanorods were conjugated to mPEG-SH 5000 and injected into mice both intravenously and subcutaneously. |
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※生物醫學領域的應用：光熱反應
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S Yoo, S Hong,Y Choi, J Park, Y Nam.
Photothermal Inhibition of Neural Activity with Near-Infrared-ensitive Nanotransducers. ACS-Nano, 2014,8(8):8040–8049. 2# |
※生物醫學領域的應用：靶向治療
Nannan Wang,Zilong Zhao, Yifan Lv al et.
Gold nanorod-photosensitizer conjugate with extracellular pH-driven tumor targeting ability for photothermal/photodynamic therapy. DOI: 10.1007/s12274-014-0493-0.
Gold nanorod-photosensitizer conjugate with extracellular pH-driven tumor targeting ability for photothermal/photodynamic therapy. DOI: 10.1007/s12274-014-0493-0.
| Preparation of Au nanorods (AuNRs) :
AuNRs were prepared according to the seed-mediated protocol with CTAB as a template. First, gold seeds were prepared by rapidly adding 0.45 mL ice-cold NaBH 4 (0.01 M) to the mixed solution of 0.625 mL HAuCl 4 (2 mM), 1.88 mL CTAB (0.2 M) and 1.37 mL distilled water, followed by rapid stirring for 2 min. During the stirring, the color of the mixture rapidly changed from bright brown-yellow to pale brown-yellow, indicating the reduction of Au 3+ to Au. Then, the solution was kept in a water bath at 25 °C. After 2 h, this seed solution could be used in growth solution. Growth solution in a volume of 20.0 mL was prepared by mixing 4 mL HAuCl 4 (2 mM), 9.5 mL CTAB (0.2 M) and 6.2 mL distilled water, followed by the addition of 0.12 mL AgNO 3 (0.01 M) and 0.128 mL AA (0.1 M). Upon adding and mixing AA, the color of the mixed solution immediately changed to colorless from bright brown-yellow. Finally, 0.086 mL of as-prepared gold seeds solution was added to the above solution and stirred vigorously for 10 min, with the color gradually becoming dark pink. The mixed solution was left undisturbed overnight for further growth. The UV-vis spectrum of as-synthesized AuNRs was monitored using a UV spectrophotometer (UV-2450, Shimadzu). The size of AuNRs was analyzed with a JEOL JEM-2100F transmission electron microscope. The concentration of AuNRs was calculated according to a previous report [42]. |
 [42] Zhou, R.; Zhou, H. Y.; Xiong, B.; He, Y.; Yeung, E. S.; Pericellular matrix enhances retention and cellular uptake of nanoparticles, J. Am. Chem. Soc. 2012, 134, 13404-13409. |